Fig 2. TSPO protein expression is higher in MDM than in monocytes.

TSPO protein was assessed by Western blot with β-actin as loading control (Ai), with accompanying densitometry normalised to β-actin (Aii). Results shown are for THP-1 monocytes, healthy peripheral blood monocytes, synovial fluid monocytes, and corresponding MDM. (B) [³H]PBR28 tracer binding per 1x10⁶ cells in healthy human peripheral blood monocytes and MDM. Each experiment was run in triplicate, and data expressed as mean± SEM for five independent experiments. *** p<0.001 using Student’s t-test, comparing data from each MDM with that of their counterpart monocytes.