Fig 7. Western blotting indicates TSPO protein expression is largely unchanged in MDM treated with M2 stimuli.
(i) representative Western blots of TSPO protein with β-actin acting as loading control and (ii) densitometry normalized to β-actin in (A) THP-1 MDM, (B) healthy peripheral blood MDM and (C) synovial fluid MDM either unstimulated (Unstim) treated with 20ng/mL IL-4, 500nM dexamethasone or 3ng/mL TGF-β1 for 24 hours. Densitometry data is expressed as the mean of five independent experiments ± SEM. (*p<0.05, **p <0.01, ***p≤0.001).