Fig. 4.

In vitro cytotoxicity of CSIC at various concentrations against HEC-1A and J774.A1 cell lines. The cytotoxicity of CSIC was measured at concentrations ranging from 10 μg/mL to 1 ng/mL. Following 24 h drug treatment, cell viability was determined by MTT assay. The absorbance was measured at 595 nm using a microplate reader (Beckman Coulter DTX 880) (n=6). The 50% cytotoxic concentrations (CC₅₀) of CSIC, for both cell lines, were found to be greater than 5 μg/mL