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MS data of (A)apo- (B) Zn2+ bound (purple star) (C) Fe2+ bound (green star) HDAC8 incubated with PCBP1. Hetero-dimer signals for the HDAC8 - PCBP1 complex are detected in all cases, independent of metal substitution. A quantitative KD analysis suggests that, although within error, iron may subtly promote HDAC8 binding to PCBP1.
