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The binding affinity (KD) values for HDAC8 - PCBP1 complex measured by direct ESI-MS method.
| HDAC8 conc. (μM)* | PCBP1 conc. (μM)* | KD (μM) |
|---|---|---|
| 21 | ||
| 6 | 13 | 28 |
| 29 | ||
|
| ||
| 60 | ||
| 10 | 10 | 82 |
| 72 | ||
|
| ||
| 56 | ||
| 18 | 15 | 59 |
| 63 | ||
|
| ||
| 78 | ||
| 5 | 5.5 | 101 |
| 112 | ||
Twelve measurements (four different molar ratios, each with three replicas) are presented. Protein concentrations are measured by UV-Vis Spectroscopy directly from buffer exchanged solution.
