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GFP was detected 3 months after induction of 2-month-old double transgenic mice. (A, B) GFP was detected in corneal stroma cells of double transgenic mice after 3 months of Dox induction from P60 to P62. (C–E) Images taken from the central cornea of the same section shown in (A) and (B). Red fluorescence showing keratocan expression in the stromal cells of the double transgenic mice (B, D, E). Note that a very low ratio of corneal stroma keratocytes lacked GFP (C–E, arrows). Ir, iris.
