Skip to main content
. 2017 Sep 6;6:e27362. doi: 10.7554/eLife.27362

3. Photobleaching assay for tracking secretory cargo.

Download video file (6.9MB, mp4)
DOI: 10.7554/eLife.27362.026

Photobleaching assay in a neuron expressing GFP-Rab11 and 3xFM-mCh-GluA1 150’ following ER release. Left panel shows GFP-Rab11, middle shows 3xFM-mCh-GluA1 and right shows the merge. Rapid (4 Hz), single plane imaging was carried out before and after photobleaching. Scale bar, 20 μm.