Glutathione (GSH-t), protein thiol (PSH) levels, and system xc− activity in HT22 cells exposed to MGO. (A) Glutathione, (B) system xc− activity (glutamate uptake,) and (C) PSH levels were determined in HT22 cells exposed to MGO 0.3 or 0.75 mM for the indicated periods. Values were normalized as percentage of untreated cells and presented as means + SEM. Sample size were N=5–9 for GSH-t and PSH, and N=3–4 for system Xc−. P <0.05 (*), 0.01 (**), or 0.001 (***) are indicated relative to untreated control (dashed line).