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. Author manuscript; available in PMC: 2017 Oct 2.
Published in final edited form as: Cell Rep. 2017 Sep 5;20(10):2480–2489. doi: 10.1016/j.celrep.2017.08.050

Figure 6. Loss of VGF impairs sustained insulin secretion.

Figure 6

Islets were isolated from control (vehicle) and β-cell VGF KO (tamoxifen) mice as indicated in the experimental methods section. Insulin secretion profiles of perifused islets following stabilization at basal (2.5 mM) glucose are shown. (A) Islets were stimulated for 16 min at 16.7 mM Glc. (B) Islets were stimulated for 12 min with KCl (35 mM) at basal (2.5 mM) glucose followed by 12 min at basal glucose in the absence of KCl and repeated twice. (C, D) Islets were stimulated with a cocktail containing 11.2 mM Glc, 100 μM diazoxide (Dz), and 35 mM KCl for the indicated times. Data represent the mean ± S.E.M. (n = 4–6 mice per treatment). p < 0.05 tamoxifen vs. vehicle control.