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. Author manuscript; available in PMC: 2018 Oct 2.
Published in final edited form as: Mol Pharm. 2017 Sep 18;14(10):3269–3280. doi: 10.1021/acs.molpharmaceut.7b00322

Figure 6. High pH prevents −18 sfGFP aggregation under low sucrose conditions.

Figure 6

Viscosity-concentration curves for −18 sfGFP (pI = 5.1) in low sucrose conditions with an elevated pH. The protein was dialyzed against 10 mM tris, 146 mM sucrose, pH 8.0 buffer and concentrated by centrifugation to ~216 mg/ml. A, The absolute and B, relative viscosity-concentration dilution curves are shown as solid black icons with black dashed lines to guide the eye. High sucrose-high trehalose data with the modified Mooney fit from Figure 1B is shown for reference in hollow red icons.