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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Protein Expr Purif. 2017 Aug 24;141:1–6. doi: 10.1016/j.pep.2017.08.009

Figure 1. Representative purification progression.

Figure 1

Approximately 10 μg total E. coli lysate containing recombinant KDAC8-His6 (lane 2), 1.0 μg total protein following the first column (lane 3), and 1.0 μg total protein after the second column (lane 4) are shown on a 12.5% SDS-PAGE gel. KDAC8-His6 is apparent at 43.5 kDa in lane 3, and KDAC8 at 42.5 kDa in lane 4. KDACs were not obviously apparent in the cell lysate from E. coli or Sf9 cells.