. Author manuscript; available in PMC: 2019 Jan 1.

Published in final edited form as: Protein Expr Purif. 2017 Aug 24;141:1–6. doi: 10.1016/j.pep.2017.08.009

Table 2.

Comparison of activity of purified KDAC6-His₆ with commercially obtained KDAC6-GST with peptide substrates.

Peptide sequence	KDAC6-His6 activity (pmol min⁻¹ μg⁻¹)^a	KDAC6-GST activity (pmol min⁻¹ μg⁻¹)
ac-{K-ac}-am	4.8 ± 1.1	8.9 ± 4.1
ac-{K-ac}W-am	33.2 ± 13.7	30.9 ± 7.6
ac-RG{K-ac}-am	9.7 ± 5.1	12.2 ± 2.7
ac-RG{K-ac}W-am	45.2 ± 13.5	40.1 ± 5.9
ac-RH{K-ac}-{K-ac}-am	11.4 ± 3.7	8.7 ± 2.5
ac-RH{K-ac}-{K-ac}W-am	39.5 ± 18.7	29.7 ± 3.6

Previously reported.[33]