Figure 4.

Syk is essential for forming the inflammatory environment. (a) The accumulation of neutrophils in the ear upon anti-C7 injection was determined by flow cytometry. (b, c) Mixed bone marrow chimeras with CD45.1-expressing WT and CD45.2-expressing WT or Syk^–/– hematopoietic cells were injected with anti-C7 antibodies and neutrophil accumulation was determined by flow cytometric analysis. In panel b, each dot represents an individual ear, and panel c shows the relative migration of WT and Syk^–/– neutrophils. (d–f) Decreased in vivo CXCL2, IL-1β, and LTB₄ levels in the ears of Syk-deficient chimeras. Graphs represent mean and standard error of the mean from (a) 3 to 5 or (c) 2 to 6 mice and (d–f) 3 to 4 control or 5 to 7 anti-C7–treated mice per group from 2 to 3 independent experiments. See the text for actual P-values. C7, collagen type VII; LTB₄, leukotriene B₄; PMN, polymorphonuclear cell; WT, wild-type.