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. 2017 Oct;137(10):2131–2139. doi: 10.1016/j.jid.2017.05.017

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Syk is required for neutrophil responses on immobilized His-C7‒anti-C7 immune complexes. (a) Respiratory burst, (b) cell spreading, (c) CXCL2, and (d) LTB₄ release of WT and Syk^‒/‒neutrophils. Curves in panel a show mean and standard error of the mean of 3 independent experiments; control data points were subtracted. Panel b is representative of 3 independent experiments. Graphs in c and d show mean and standard error of the mean from 3 independent experiments. See the text for actual P-values. Scale bar = 40 μm. C7, collagen type VII; His-C7, His-tagged fragment of the immunodominant NC1 domain of murine C7; LTB₄, leukotriene B₄; min, minute; WT, wild-type.