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. 2017 Oct 2;7:12504. doi: 10.1038/s41598-017-12249-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miRNA identification, distribution and differential regulation. Image (a) presents the hairpin structure of miR172f as an example of identification of miRNAs from N. tabacum k326. The mature miRNA sequence is presented in red. The lines above or under the mature miRNA represent the matching reads in miRNA sequencing data, and the numbers represent the count of aligned reads. Image (b) presents the length distribution of miRNAs. Image (c) presents the distribution of differentially expressed miRNAs.