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. 2017 Oct 2;7:12525. doi: 10.1038/s41598-017-12979-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum Likelihood tree of the representative pahAc of the dominant genotype in CY-1, the full length pahAc gene in the newly-cloned hpah gene cluster and other representative nah-related Ac genes, built with predicted amino acid sequences. Several characterized pahAcs from Alteromonas sp. SN2, Burkerholderia sp. RP007, Sphingomonas sp. B1 and Rhodococcus sp. NCIB12308 were used as outgroup. PahAc sequences cloned in this study are shown in bold. The five types of the nah-related pahAcs are labeled in the tree, with the group’s name shown in the right of the tree.