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. 2017 Oct 2;7:12532. doi: 10.1038/s41598-017-12844-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The influence of C-C mismatch position on yield of RNase H cleavage of RNA 692. T1 – RNase T1 ladder, C- control RNA (without ASO), 15–20 – gapmers b15-b20, M- gapmer bKM complementary to mutant RNA 692, order of the gapmers on gel corresponds with changing C-dC mismatch position along the gap. b15 – C-dC at position 3/13 from 5′ end of the gapmer, b20 – C-dC at position 10/13 from 5′ end of the gapmer. This gel image was cropped and rearranged to present data of interest. Original gel image is included in Supplementary Materials as Fig. S14.