Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 2;7:12532. doi: 10.1038/s41598-017-12844-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comprehensive results of in vitro activity of gapmers designed to SNCA RNA 46 and RNA 53 targets. (A) Target SNCA mRNA sequence containing sites of two G-to-A SNPs (RNA46 and RNA53) and designed to them antisense gapmers. SNPs in RNA strand are bolded and underlined, codons in which they occur are marked by red frame. Modified nucleotides within gapmers are marked by colours: blue-LNA, green-2′-O-MeRNA, bolded black- DNA, red-mismatched nucleotides, underlined-SNP site. (B) RNase H in vitro assay results for SNCA RNA46 target, showing the influence of arrangements of mismatched nucleotides in RNA/ASO duplexes on RNase H cleavage. Blue frames mark gapmers which cause selective cleavage of Mut RNA, red frames mark gapmers which preferentially cause wild type RNA cleavage. (C) RNase H in vitro assay results for SNCA RNA53 target, showing the influence of arrangements of mismatched nucleotides in RNA/ASO duplexes on RNase H cleavage. Blue frames mark gapmers which cause selective cleavage of Mut RNA, red frames mark gapmers which preferentially cause wild type RNA cleavage. (D) Thermodynamic parameters of SNCA wild type and mutants RNA/gapmer duplexes containing mismatches. Oligonucleotides, which differentiated two alleles cleavage yields in RNase H assay were only measured. Parameters for more stable duplex of the two (wild type/ASO or mutant/ASO) are bolded.