Table 1.
Detection of ALK rearrangements in clinical specimens using IHC, FISH, AmpliSeq RNA Fusion kit, and Archer® FusionPlex® Kit.
| Sample ID | IHC results | FISH results | NGS results | Results summary | |
|---|---|---|---|---|---|
| AmpliSeq RNA Fusion kit | Archer® FusionPlex® kit | ||||
| S01 | Negative | Negative | Negative | Negative | Concordance |
| S02 | Negative | Negative | Negative | Negative | Concordance |
| S03 | Negative | Negative | Negative | Negative | Concordance |
| S04 | Negative | Negative | Negative | Negative | Concordance |
| S05 | Negative | Negative | Negative | Negative | Concordance |
| S06 | Negative | Negative | Negative | Negative | Concordance |
| S07 | Negative | Negative | Negative | Negative | Concordance |
| S08 | Negative | Negative | Negative | Negative | Concordance |
| S09 | Negative | Negative | Negative | Negative | Concordance |
| S10 | Negative | Negative | Negative | Negative | Concordance |
| S11 | Negative | Negative | Negative | Negative | Concordance |
| S12 | Negative | Negative | Negative | Negative | Concordance |
| S13 | Negative | Negative | Negative | Negative | Concordance |
| S14 | Positive | Positive | EML4-ALK (E6:A20) | EML4-ALK (E6:A20) | Concordance |
| S15 | Positive | Positive | EML4-ALK (E6:A20) | EML4-ALK (E6:A20) | Concordance |
| S16 | Positive | Positive | EML4-ALK (E6:A20) | EML4-ALK (E6:A20) | Concordance |
| S17 | Positive | Positive | EML4-ALK (E6:A20) | EML4-ALK (E6:A20) | Concordance |
| S18 | Positive | Positive | EML4-ALK (E6:A20) | EML4-ALK (E6:A20) | Concordance |
| S19 | Positive | Positive | EML4-ALK (E13:A20) | EML4-ALK (E13:A20) | Concordance |
| S20 | Positive | Positive | EML4-ALK (E13:A20) | EML4-ALK (E13:A20) | Concordance |
| S21 | Positive | Positive | EML4-ALK (E13:A20) | EML4-ALK (E13:A20) | Concordance |
| S22 | Positive | Positive | EML4-ALK (E13:A20) | EML4-ALK (E13:A20) | Concordance |
| S23 | Positive | Positive | EML4-ALK (E13:A20) | EML4-ALK (E13:A20) | Concordance |
| S24 | Positive | Positive | EML4-ALK (E13:A19) | EML4-ALK (E13:A20) | Discordance in the variant detected by NGS approaches |
| S25 | Positive | Positive | EML4-ALK (E13:A20) | EML4-ALK (E13:A20) | Concordance |
| S26 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S27 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S28 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S29 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S30 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S31 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S32 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S33 | Negative | Negative | Uncertain ALK | Negative | Discordance between NGS results |
| S34 | Negative | Negative | Uncertain RET | Negative | Discordance between NGS results |
| S35 | Positive | Positive | Uncertain ALK | GCC2-ALK (G19:A20) | Discordance between NGS results |
| S36 | Positive | Positive | Uncertain ALK | DCTN1-ALK (D26:A20) | Discordance between NGS results |
| S37 | Positive | Positive | Negative | CLIP1-ALK (C22:A20) | Discordance between NGS results |