Fig. 2.

Characterization of EV71-neutralizing mAbs. Immunoprecipitated EV71 capsids were detected by immunoblot analysis using the anti-VP0/VP2 monoclonal antibody MAB979 (1:1000 dilution, EMD Millipore) and the anti-VP1 monoclonal antibody MAB1255-M05 (1:1000 dilution, Abnova). The 12-96015 EV71-containing and mock-infected RD cell supernatants were prepared as antigens and immunoprecipitated with the anti-EV71 mAbs. The human anti-influenza monoclonal antibody 2-12C¹⁹ was included as an antibody control. For the mock control, all antibodies were set up and showed equivalent results. In the figure, one mock control is shown