Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 2;7:12480. doi: 10.1038/s41598-017-12662-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mitochondrial content and biogenesis. (a,b,f,g) Immunoblot analysis of the subunits of mitochondrial complexes in MEF cells (n = 5; df = 4) (a,b) and in liver (n = 5; df = 8) (f,g). (b,g) Quantifications of Cmpl. I-V protein levels from (A) MEFs and (f) liver, respectively. Cmpl. I (NDUFB8, NADH dehydrogenase 1 beta subcomplex subunit 8) (MEFs: t = 4.981; Liver: t = 1.17); Cmpl. II: SDHB (Succinate dehydrogenase iron-sulfur subunit) (MEFs: t = 3.685; Liver: t: 2.23); Cmpl. III: UQCRC2 (Cytochrome b-c1 complex subunit 2) (MEFs: t = 5.411; Liver: 2.383); Cmpl. IV: MTCO1 (Cytochrome c oxidase subunit 1) (MEFs: t = 2.378); COXIV (Cytochrome c oxidase subunit 4) (Liver: t = 4.79); Cmpl. V: ATP5A (ATP synthase subunit alpha) (MEFs: t = 2.302; Liver: t = 1.806). (c,h) Analysis of mtDNA content number by qPCR in MEF cells (n = 4; t = 3.544; df = 3) (c) and liver (n = 5; t = 1.944; df = 8) (h). The mtDNA content was calculated as the mtDNA/nDNA ratio using primers specific for the mitochondrial TrnL1and nuclear β-2-microglobulin (β2M) genes. (d) Immunoblot analysis of PGC1α in MEFs (n = 7; t = 5.764; df = 6). (e) Quantification of PGC1α protein levels from (d) MEFs. (i) Quantification of PGC1α protein level in the liver of WT and Rev1^−/− 5 months old mice (t = 1.761). n = sample number; t = t value; df = degree of freedom; *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. Data presented are mean ± M.S.E.