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. 2017 Oct 2;7:12535. doi: 10.1038/s41598-017-12082-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Modulation of LPS-induced iNOS expression by compound 10 in BV-2 microglial cells. BV2 cells were pretreated with the compound for 30 min and then stimulated with 100 ng/mL of LPS for 6 h. The cell lysates were extracted, and the protein levels of iNOS were analysed by Western blotting. (A) iNOS expression in LPS-activated BV2 cells. (B) Densitometric analysis of iNOS expression. α-Tubulin was used as the loading control. The figures show the representative results of three independent experiments. ^### P < 0.001 compared to the untreated control, *P < 0.05, and ***P < 0.001 compared to the LPS-treated group.