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. 2017 Oct 2;7:12540. doi: 10.1038/s41598-017-12895-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction relationships among H₂O₂, NO, and SA (a,b, and c) and the effects of these signals on fungus-induced salidroside and tyrosol accumulations (d) in R. crenulata plantlets inoculated with endophytic fungus ZPRs-R11. NO was a key node signal molecule. NO and SA exhibited remarkable activities in accumulation of the two metabolites. The working concentration of SNP, SA, H₂O₂, and CA was 1 mM, and the cPTIO and CAT concentrations were 30 μM and 2 mkat L⁻¹, respectively. SNP: sodium nitroprusside; SA: salicylic acid, H₂O₂: hydrogen peroxide; CA: trans-cinnamic acid; cPTIO: 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt; CAT: catalase; FW: fresh weight. Plantlets were treated on the 10th day. Values were the means of three repeats ± standard error.