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. 2017 Oct 2;7:12539. doi: 10.1038/s41598-017-12845-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amino acid sequence alignments of three Fasciclin like arabinogalactan polypeptide sequences TC379245, DQ872381 and, Traes_2BS_D44E4B43A. For TC379245 (Triticum Aestivum Gene Indices), DQ872381 was the top hit on NCBI BLAST and Traes_2BS_D44E4B43A on Ensembl BLAST. Red boxes indicate the AGP GLyco-modules comprising of Alanine and Proline. Black boxes indicate amino acids comprising the FAS domain comprising of H1, [YF]H and H2 conserved regions. Red dots indicate conflict positions. Multiway sequence alignment was carried out using Clone Manager (Sci Ed, USA). Note – TC379245 ORF was corrected (‘C’ at position 425 bp removed) before translating the nucleotide sequence.