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. 2017 Oct 2;7:12539. doi: 10.1038/s41598-017-12845-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tissue-specific expression (RPKM) of FLA transcripts in developing wheat seeds. The mean of three biological replicates²⁰ is presented for different tissues of the developing wheat grain at 12 days-post-anthesis in the cultivar, Holdfast. ESP: Endosperm; IP: inner pericarp; OP: outer pericarp; TC378245 and GH726097: transcript IDs correspond to FLA genes from the TAGI database Raw reads obtained from data reported by Pearce et al. (2015)²⁰ (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3103/samples/) were processed by RNA-Seq analysis to deduce FLA expression, using CLCbio Workbench (Qiagen Bioinformatics, Denmark).