Table 2.

Definition of morphological classes for the flow cytometry method vs. image analysis

	Microscopy	Flow cytometry
Hyphae	• Area bigger than 100 μm2:   → When a hyphal diameter of 3 μm is assumed, this means that the minimal length of hyphae is 33.3 μm. • Area smaller than 3500 μm2 • Skeletonized pictures do not form a loop (Paul and Thomas 1998)	• Sample length between 10 and 150 μm • SWS total smaller than 4∙104 • FWS length below 190 μm   → Exact ratios of SWS total and FWS length see in Fig.1c
Small clumps	• Area in-between 1000 and 3500 μm2 • Skeletonized pictures form a loop (Paul and Thomas 1998).	• Sample length between 10 and 150 μm • SWS total in-between 1.8∙104 and 9∙104 • FWS length higher than 18 μm   → Exact ratios of SWS total and FWS length see in Fig. 1c.
Large clumps	• Area bigger than 3500 μm2 • Hyphal aggregate not classified as pellet	• SWS total higher than 2∙104 and sample length bigger than 100 μm • Is not a pellet
Pellet	• Area bigger than 7500 μm2 • Has a core • Core area bigger than 7000 μm2	• SWS total higher than 4∙104 and sample length bigger than 120 μm • A core exists (saturated FWS) • Ratio of FWS maximal: FWS fill factor bigger than 7.5∙103 • SWS total:sample length bigger than 8∙102