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. 2017 Sep 28;4:160. doi: 10.3389/fvets.2017.00160

Figure 3.

Figure 3

Misoprostol treatment 30 and 60 min following lipopolysaccharide (LPS) stimulation inhibits tumor necrosis factor α (TNFα), IL-6, and IL-1β mRNA production in equine leukocytes. Equine leukocyte-rich plasma was stimulated with 100 ng/mL LPS or vehicle (sterile PBS), followed 30 or 60 min later by treatment with 100 µM misoprostol or vehicle (0.05% DMSO). Cells were incubated for (A) 2 h or (B) 6 h, and mRNA was isolated. Levels of TNFα, IL-6, and IL-1β mRNA were assessed via real-time PCR with each sample assayed in triplicate. Data are expressed as mean ± SEM fold change in mRNA levels versus LPS-stimulated cells that were posttreated with the misoprostol vehicle (denoted as 0 µM misoprostol). **p < 0.001 and *p < 0.05 indicate a significant deference compared to LPS-stimulated cells that were posttreated with the misoprostol vehicle (black bars) via one-way RM ANOVA; n = 6 [(A), 2 h incubation] or n = 4 [(B), 6 h incubation].