Figure 1.

Structure determination and overall structure of Sizzled. A, initial 2F_o − F_c electron density map (contour level, 1.2 σ; curve parameter, 1.6 Å) calculated with experimental phases derived from three sulfurs (yellow spheres). B, ribbon model of overall structure of Sizzled with the inter-domain linker (residues 143–154) represented by a dotted line and disulfide bonds by sticks. C, residues distributed on the CRD-NTR interface are shown by stick representation. The F_o − F_c electron density map (contour level, 3.0 σ) was calculated with phases derived from the final model except for these residues. The purple dashed line indicates the hydrogen bond.