Figure 6.
Crystal packing of Sizzled CRD and MALS analysis of Sizzled. A, crystal packing pattern of Sizzled (CRD in cyan, NTR in salmon, and linker in dashed line) with four symmetry mates presented. The NTR domain of sym-3 (or sym-2) docks into the concave surface formed by NTR and CRD of sym-1 (or sym-4), burying 1900 Å2 total surface area. The C-terminal portion of CRD is indicated by a red sphere. B, MALS analysis of Sizzled in solution. The brown dashed line across the refractive index peak (RI, blue dashed line) and the light scattering peak (LS, red line) indicates homogeneity of Sizzled. The calculated apparent molecular mass is 32.3 ± 0.4 kDa. The right vertical axis represents refractive index. For clarity, the vertical axis for light scattering intensity was not shown.