Table 1.
Kinetic parameters describing the interactions of PfCRTDd2 and PfCRTHB3 with ferrous and ferric iron
Best-fit values of kinetic parameters were obtained from the kinetics of ferrous and ferric iron uptake using the Michaelis–Menten equation (Fig. 8). The means ± S.E. of NBR (shown in parentheses) independent biological determinations are shown. Statistical significance was determined between corresponding kinetic parameters, using a two-tailed t test.
| Iron | Parameters | PfCRTHB3 | PfCRTDd2 | p |
|---|---|---|---|---|
| Fe2+ | Apparent Vmax (pmol oocyte−1 h−1) | 13 ± 1 (5) | 4 ± 0.5 (5) | <0.001 |
| Apparent Km (μm) | 70 ± 17 (5) | 20 ± 9 (5) | 0.02 | |
| Fe3+ | Apparent Vmax (pmol oocyte−1 h−1) | 20 ± 2 (5) | 14 ± 2 (5) | 0.05 |
| Apparent Km (μm) | 130 ± 40 (5) | 170 ± 50 (5) | 0.5 |