Figure 1.

The hMEX-3C KH domains bind the MRE10 RNA. A, sequence alignment of the KH domains of human MEX-3C and C. elegans MEX-3. Conserved residues are highlighted in gray. The GXXG motif is indicated by a red dashed box. The secondary structure elements of human MEX-3C KH domain assigned on the basis of the X-ray structure are shown at the top. B, the domain architecture of human MEX-3C and the sequence of MRE10 RNA. The black lines represent the hMEX-3C constructs used for ITC and structure determination. C, the ITC fitting result of hMEX-3C KH1/2 domains with MRE10 RNA. D, the ITC fitting results of individual hMEX-3C KH domain with MRE10a or MRE10b.