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. 2017 Aug 21;292(39):16267–16276. doi: 10.1074/jbc.M116.763680

Figure 7.

Figure 7.

Cdc6 regulates the amounts of PCM proteins at the centrosome. Asynchronously grown U2OS Tet-On inducible cell lines used and induction of the indicated proteins are shown in Figs. 2 and 4. A, cells were co-immunostained with the indicated antibodies. Fields around the centrosomes containing 4 dots of centrin are shown at higher magnifications in insets. Scale bar, 10 μm. B, ratios are described as relative intensities of the indicated protein to relative intensity of centrin. PCNT, pericentrin; Tet-Ctrl, control U2OS Tet-On cells; WT, FLAG-Cdc6 full-length wild type; LI/AA, FLAG-Cdc6(LI/AA). C, Tet-Ctrl, U2OS Tet-On control cells; WT, FLAG-Cdc6(75–366) wild type; K208A, FLAG-Cdc6(75–366)(K208A). Values represent mean ± S.D. of at least 50 cells in three independent experiments. D, Cdc6 negatively controls PCM assembly and microtubule nucleation.