Table 1.
HPLC analysis of porphyrins from control zebrafish embryos and heme synthetic morphants
Pooled zebrafish embryos were analyzed as described under “Experimental procedures.” Significant changes (p ≤ 0.05) compared with uninjected controls are shown in boldface type and underlined.
| Hemin | S.D. hemin | PPIX | S.D. PPIX | ZnPPIX | S.D. ZnPPIX | Intermediate porphyrins | S.D. intermediate porphyrins | |
|---|---|---|---|---|---|---|---|---|
| pmol/mg | pmol/mg | pmol/mg | pmol/mg | |||||
| Uninjected controls | 648.0 | 36.0 | Trace | Trace | 1.4 | 0.3 | ||
| 0.19 mm abcb10 MO2 | 238.0 | 12.2 | Trace | Trace | 1.0 | 0.4 | ||
| Uninjected controls | 477.4 | 47.9 | 1.5 | 0.3 | Trace | Trace | ||
| 0.75 mm abcb10 MO3 | 77.5 | 30.5 | 1.9 | 0.6 | Trace | Trace | ||
| Uninjected controls | 237 | 11.4 | 1.2 | 0.3 | Trace | Trace | ||
| 0.175 mm urod MO | Trace | 14.5 | 2.1 | Trace | 51 | 24 | ||
| Uninjected controls | 726.5 | 60 | 2 | 0.9 | Trace | Trace | ||
| 0.75 mm fech MO | 102.3 | 17.1 | 518.4 | 98 | 32.4 | 3.8 | Trace |