Figure 5.

Cross-link map of Ecm29-proteasome interactions. Diagram of unique Lys–Lys linkages between Ecm29 and the 19S base subunits Rpn1, Rpn10, Rpt1, Rpt4, and Rpt5. The remaining members of the AAA-ATPase ring are also included for spatial context. Cross-linked Ecm29 residues are labeled in green, whereas the residues of the respective 19S RP subunits are shown in blue. Various functional domains of individual proteins are labeled according to SMART: coiled-coil domain, orange; AAA-ATPase, blue; von Willebrand factor (VWA), green; ubiquitin-interacting motif (UIM), magenta; HEAT repeats, brown. The T1 and T2 domains of Rpn1 are shown in yellow and orange, respectively.