Figure 4.

PKC activity is required for the ability of syndecan-2 to up-regulate MMP-7. A and B, HT-29 cells stably expressing syndecan-2 or the ΔC31 mutant were treated with 50 ng/ml PMA or 0.1 μm GF109203X. At 6 and 24 h post-treatment, the expression levels of the target mRNAs were analyzed by RT-PCR and RT-qPCR. Data are shown as mean ± S.D. (error bars) (n = 3), normalized to GAPDH expression. *, p < 0.05. A, conditioned media were collected from the indicated cells and immunoblotted with the MMP-7–specific antibody (B). C, HT-29 cells with or without stable expression of syndecan-2 were treated with 0.1 μm GF109203X. After 24 h, the cells were seeded on soft agar and grown for 17 days. The colonies were then stained with 0.005% crystal violet and counted. Data are shown as mean ± S.D. (n = 3). *, p < 0.05.