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. 2017 Jun 21;313(3):C305–C313. doi: 10.1152/ajpcell.00005.2017

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Fig. 5. — IL-22 receptor neutralization blocks IL-22 protection from alcohol-induced injury. Mouse primary hepatocytes were isolated and cultured in a 96-well plate overnight. Primary hepatocytes treated with ethanol (100 mM) exhibited significantly enhanced caspase-3/7 activation compared with control cells as determined using Apo-ONE Homogeneous Caspase-3/7 activity kit. When hepatocytes were pretreated with recombinant IL-22, there was a significant reduction of ethanol-induced caspase activation (*P < 0.05). IL-22 receptor-α (IL-22R1) blockage with neutralizing antibody (Ab) demonstrated that the caspase inhibitory effect of IL-22 in ethanol-treated cells was lost (n = 3; *P < 0.05).