. 2017 Jun 28;313(3):C295–C304. doi: 10.1152/ajpcell.00097.2017

Table 2.

Triacylglycerol fatty acid composition in inguinal and epididymal white adipose tissue

FA, μg/mg tissue	iWAT			eWAT
FA, μg/mg tissue	WT	HET	KO	WT	HET	KO
12:00	97.27 ± 15.58	81.01 ± 17.19	59.19 ± 7.36	134.90 ± 30.96^ab	118.22 ± 6.24^a	47.61 ± 9.28^b
14:00	574.95 ± 77.37	536.01 ± 130.69	827.44 ± 118.29	1239.57 ± 95.72^a	940.05 ± 94.76^b	859.16 ± 135.11^b
15:00	74.02 ± 5.92	130.62 ± 25.60	94.39 ± 9.84	183.42 ± 28.31	129.43 ± 14.77	112.05 ± 12.53
16:00	7,174.85 ± 811.27	11,007.89 ± 2,304.68	12,213.35 ± 1,337.55	22,575.92 ± 2,827.07	18,417.50 ± 2405.07	16,077.70 ± 2,878.11
18:00	683.63 ± 105.77^a	1,100.62 ± 312.06^a	8,809.89 ± 1,232.07^b	1,883.15 ± 283.19^a	1,786.34 ± 214.47^a	7,078.99 ± 1,143.10^b
20:00	35.60 ± 3.55^a	43.89 ± 9.83^a	120.10 ± 10.60^b	66.94 ± 5.45	60.67 ± 6.75	106.52 ± 20.70
24:00:00	38.54 ± 5.96^a	103.12 ± 14.56^b	36.04 ± 7.50^a	189.09 ± 48.86	106.96 ± 33.18	61.80 ± 13.88
Total SFA	86,78.86 ± 1,025.42^a	13,003.16 ± 2,814.61^a	22,160.4 ± 2,723.21^b	26,272.99 ± 3,319.56	21,559.16 ± 2,775.24	24,343.83 ± 4,212.71
14:1c9	122.32 ± 16.20	160.96 ± 24.96	ND	117.99 ± 25.30^ab	136.35 ± 6.71^a	60.49 ± 9.10^b
16:1n7	2,362.10 ± 285.23^a	2,143.09 ± 593.12^a	558.18 ± 61.03^b	6,752.46 ± 996.74^a	3,314.69 ± 424.35^b	794.00 ± 105.15^c
18:1n9	10,038.61 ± 1,202.41	8,391.72 ± 2148.11	9,086.70 ± 803.33	28,379.21 ± 5539.91^a	23,097.61 ± 3125.65^a	11,133.08 ± 1,384.33^b
18:1c11	711.60 ± 88.92	1,094.50 ± 240.43	748.44 ± 90.27	2,286.04 ± 368.14^a	1300.45 ± 224.29^b	778.86 ± 124.1^b
20:1c11	513.25 ± 48.24	385.29 ± 106.72	588.66 ± 73.51	1,062.98 ± 152.21^a	859.09 ± 135.43^a	618.31 ± 101.38^b
22:1n9	82.52 ± 11.49	97.42 ± 30.87	162.05 ± 27.18	58.04 ± 7.76^a	67.60 ± 15.02^a	33.42 ± 5.17^b
Total MUFA	13,830.4 ± 1,652.49	12,272.98 ± 3,144.21	11,144.03 ± 1,055.32	38,656.72 ± 7,090.06^a	28,775.79 ± 3,931.45^a	13,418.16 ± 1,729.23^b
18:2n6	7,422.26 ± 981.78	10,971.98 ± 2,513.73	10,132.61 ± 1,202.13	21,248.24 ± 3,916.41	15,978.86 ± 2,713.67	15,017.38 ± 2,120.03
18:3n6	52.73 ± 5.22	70.41 ± 13.21	53.15 ± 8.32	140.68 ± 27.87	97.24 ± 13.03	75.07 ± 11.51
20:2n6	67.42 ± 9.20	39.11 ± 8.42	49.60 ± 9.29	165.00 ± 20.42^a	62.72 ± 21.43^b	102.87 ± 10.57^b
20:3n6	113.27 ± 18.93	125.87 ± 25.70	80.33 ± 12.18	200.55 ± 32.18^a	157.32 ± 24.48^a	90.54 ± 11.09^b
20:4n6	83.51 ± 10.95	103.59 ± 28.01	81.03 ± 12.56	196.50 ± 57.54	175.37 ± 33.66	100.33 ± 15.41
22:4n6	69.05 ± 9.97	91.80 ± 16.90	ND	97.95 ± 20.85^a	93.75 ± 16.44^a	37.64 ± 8.17^b
Total n-6	7,808.24 ± 1,036.05	11,402.76 ± 2,605.97	10,396.72 ± 1,244.48	22,048.92 ± 4,075.27	16,565.26 ± 2,822.71	15,423.83 ± 2,176.78
18:3n3	376.80 ± 44.95	548.99 ± 127.40	540.39 ± 56.59	1,091.10 ± 184.72	915.42 ± 114.14	784.29 ± 97.58
18:4n3	63.18 ± 11.96	82.33 ± 15.07	74.77 ± 10.75	162.52 ± 31.19^a	126.23 ± 19.51^a	80.17 ± 14.67^b
20:5n3	48.59 ± 5.63	126.44 ± 24.31	66.42 ± 10.29	172.49 ± 38.90	79.07 ± 6.42	96.31 ± 18.66
22:5n3	155.59 ± 70.59	98.86 ± 22.03	72.51 ± 7.34	164.05 ± 26.38^a	159.58 ± 21.13^a	66.73 ± 11.57^b
22:6n3	101.31 ± 12.00	140.74 ± 38.12	145.14 ± 16.13	271.34 ± 36.41	202.78 ± 40.46	131.67 ± 30.53
Total n-3	745.47 ± 145.13	997.36 ± 226.93	899.23 ± 101.1	1,861.49 ± 317.60	1,483.09 ± 201.66	1,159.18 ± 173.01

Data correspond to mean μg fatty acid (FA)/mg tissue ± SE (n = 6 mice per genotype). FAs were analyzed between the 3 genotypes in a white adipose tissue (WAT) depot-specific manner using a 1-way ANOVA. FAs with different letters are significantly different from one another (P < 0.05). The absence of letters means there were no significant differences for a FA in a specific WAT depot between the 3 genotypes. iWAT, inguinal white adipose tissue; eWAT, epididymal white adipose tissue; WT, wild-type mice; HET, heterozygous mice; KO, Scd1 knockout mice; ND, not detected; SFA, saturated fatty acids; MUFA, monounsaturated fatty acids.