Fig. 6.

GSK-3β rescues oxidative stress-decreased PRMT4 at protein level. Empty vector (A) or GSK-3β constructs (C) were introduced into MLE12 cells for 48 h. H₂O₂ was added into the cells at variant amounts as indicated. Cell lysates were immunoblotting analyzed with PRMT4, GSK-3β, and γ-tubulin antibodies, respectively. B and D: the densitometry results of A and C were plotted. C: GSK-3β protein were ectopically expressed in MLE12 cells for 48 h. Results are representative of 3 independent experiments. *P < 0.05 or **P ≤ 0.01 vs. corresponding control.