Fig. 1.

Bladder outlet obstruction (BOO) causes an increase in bladder weight that is blocked by glyburide and anakinra. Rats were obstructed for 12 days and given daily doses of the NLRP3 inhibitor glyburide or the IL-1β antagonist anakinra as described in materials and methods. Control, sham-operated, and BOO rats given vehicle served as controls; after 12 days, animals were killed and bladder wet weights were taken. Results are the means ± SE; n = 6, 8, 5, 5, and 5, respectively. Brackets above the graph indicate group comparisons and their level of significance. All groups were compared using a one-way ANOVA followed by a Student-Newman-Keuls post hoc test. Differences between any two groups not indicated by brackets were not significant. *P < 0.05, **P < 0.01, ***P < 0.005.