Fig. 4.

Sulfilimine cross-link content in wild-type (WT) and peroxidasin knockout (KO) mice. A: Coomassie blue-stained gels after SDS-PAGE under nonreducing conditions of purified NC1 hexamers from WT and peroxidasin KO mice. Under denaturing conditions, hexamers dissociate into singly cross-linked dimeric (D1), doubly cross-linked dimeric (D2), and un-cross-linked monomeric (M) subunits. B: number of sulfilimine cross-links per hexamer was quantified using densitometry of D1, D2, and M subunits for WT and KO mice. Individual data points (n = 4) are displayed with mean and SE. *P < 0.05 (by unpaired t-test).