Fig. 3.
Podocyte-specific knockdown of Sirt1 accelerates aging-related podocyte expression markers. A: expression of Sirt1 and podocyte-specific markers were assessed from mRNAs of isolated glomeruli in young control and in aged mice. B: glomerular Sirt1 (top) and podocyte-specific p57 expressions (bottom) are detected by immunostaining. Glomeruli are outlined with dotted lines. C and D: quantification of Sirt1 (C) and p57 (D) per glomeruli is shown. **P < 0.01 and ***P < 0.001, compared with young controls; #P < 0.05 and ###P < 0.001, compared with Pod-LuciRNAi mice (n = 6 mice in each group).