Fig. 4.

Podocyte-specific knockdown of Sirt1 increases markers of senescence in glomeruli. A: Expression of senescence markers p19ARF and p16INK4a, assessed mRNAs of isolated glomeruli, were significantly increased aged mice compared with the young controls, and their increases were further heightened in Pod-Sirt1^RNAi mice compared with Pod-Luci^RNAi mice. B: expression of pSer139-H2A.X was significantly increased in glomeruli of aged Pod-Sirt1^RNAi mice by immunohistochemical analysis. ***P < 0.001, compared with young controls; ##P < 0.01, compared with Pod-Luci^RNAi mice (n = 6 mice in each group).