Fig. 5.

Expression of de novo lipogenesis enzymes and Kennedy pathway enzymes downstream of PA are not significantly altered in epididymal or perirenal WAT from male Agpat4^−/− mice. Anaylsis of a subset of Kennedy pathway genes in epididymal (A) and perirenal (B) WAT (n = 5–7). Representative immunoblots showing content of enzyme regulators of FA de novo biosynthesis and complex lipid synthesis in epididymal (C) and perirenal (D) WAT. Quantification relative to total protein content per lane of immunoblots from epididymal (C, right panel) and perirenal (D, right panel) WATQ6 (n = 4–8). Data are means ± SEM. *P < 0.05 versus wild-type.