TABLE 1.
PCR primers
| Gene | Direction | Sequence | Product Size (bp) |
| Agpat1 | Forward | 5′-AGA CCT TGC TCA CCC AGG AT-3′ | 134 |
| Reverse | 5′-GAT GGG GAT GAT GGG GAC CT-3′ | ||
| Agpat2 | Forward | 5′-CCGTGGTGTACTCGTCTTTCT-3′ | 107 |
| Reverse | 5′-CAGACCATTGGTAGGGACAGC-3′ | ||
| Agpat3 | Forward | 5′-GCT TCG TCC TGG GTG TCT TT-3′ | 137 |
| Reverse | 5′-GTT GCC ATA GCT GGA GCC TT-3′ | ||
| Agpat5 | Forward | 5′-GGA CAT GTG CGC TAC GTA CT-3′ | 167 |
| Reverse | 5′-AGA TAC ATC GGT GTT CCT GCG-3′ | ||
| Cdipt | Forward | 5′-GATCGACCTGTCTGGGAACC-3′ | 78 |
| Reverse | 5′-TTTCCAGCACACAGGGTGAA-3′ | ||
| Cds1 | Forward | 5′-TGGACATGGCGGGATAATGG-3′ | 111 |
| Reverse | 5′-TGGAGCACTTTGCTGGGATT-3′ | ||
| Cds2 | Forward | 5′-GGGTTCTTCGCCAGTGGATT-3′ | 89 |
| Reverse | 5′-AAGCGATCCATGATGCCTCC-3′ | ||
| C/ebpα | Forward | 5′-GCAAAGCCAAGAAGTCGGTG-3′ | 114 |
| Reverse | 5′-TCTCCACGTTGCGTTGTTTG-3′ | ||
| C/ebpβ | Forward | 5′-AAGCTGAGCGACGAGTACAAGA-3′ | 116 |
| Reverse | 5′-GTCAGCTCCAGCACCTTGTG-3′ | ||
| Cept1 | Forward | 5′-TTGTACTGTGGCAGGGACCA-3′ | 97 |
| Reverse | 5′-TGTTCCTGCTATGGTTGACCC-3′ | ||
| Chpt1 | Forward | 5′-ACTGTCTTTATTGGGCCAGGT-3′ | 90 |
| Reverse | 5′-GACCATTGCTATCCACAGAACA-3′ | ||
| Dgat1 | Forward | 5′-CTGGATTGTGGGCCGATTCT-3′ | 94 |
| Reverse | 5′-ATACATGAGCACAGCCACCG-3′ | ||
| Dgat2 | Forward | 5′-AAGACATCGACCTGTACCATGC-3′ | 92 |
| Reverse | 5′-CTCAGTCTCTGGAAGGCCAAA-3′ | ||
| Fabp4 | Forward | 5′-GTGGGATGGAAAGTCGACCA-3′ | 70 |
| Reverse | 5′-CATAACACATTCCACCACCAGC-3′ | ||
| Fat/cd36 | Forward | 5′-ACTGTGGCTAAATGAGACTGGG-3′ | 93 |
| Reverse | 5′-ACCATGCCAAGGAGCTTGAT-3′ | ||
| Gpat1 | Forward | 5′-CTGCAGACGCCGCTGG-3′ | 179 |
| Reverse | 5′-AACCCACAGTCAACCCAGTC-3′ | ||
| Gpat2 | Forward | 5′-ACACTTGTTGCAGCCCATTG-3′ | 161 |
| Reverse | 5′-CCACACTGGCACTCAGAACA-3′ | ||
| Gpat3 | Forward | 5′-CACGGTGGATTGATGGGGAT-3′ | 173 |
| Reverse | 5′-TTGATGCAAGTACCTTCTGGGA-3′ | ||
| Gpat4 | Forward | 5′-GGTGGCTAAGAGGCTGACTG-3′ | 155 |
| Reverse | 5′-ACTTGATAGCCACAGGGTAAACA-3′ | ||
| Il-1β | Forward | 5′-cctgctggtgtgtgacgttccc-3′ | 84 |
| Reverse | 5′-gggtccgacagcacgaggct-3′ | ||
| Il-6 | Forward | 5′-gctggagtcacagaaggagtggct-3′ | 79 |
| Reverse | 5′-ggcataacgcactaggtttgccgag-3′ | ||
| Iplaβ | Forward | 5′-CCTTTCCATTACGCTGTGCAA-3′ | 103 |
| Reverse | 5′-GACTCACGGCTTGGTTGTT-3′ | ||
| Iplaγ | Forward | 5′-CAAAGACAAGAAGGCAGAGGAG-3′ | 106 |
| Reverse | 5′-TAAGGCCTGAACTAAGGCTCG-3′ | ||
| Lpin1 | Forward | 5′-CCTTAGGGAGCCGGAAGACT-3′ | 91 |
| Reverse | 5′-ATTGTTGGCGACTGGTCACT-3′ | ||
| Pgs1 | Forward | 5′-GGGTCCAGCTCCAGGAATAC-3′ | 111 |
| Reverse | 5′-TAGGAGAGCCAATCAGCGTG-3′ | ||
| Pparγ | Forward | 5′-CACAATGCCATCAGGTTTGG-3′ | 82 |
| Reverse | 5′-GCTGGTCGATATCACTGGAGATC-3′ | ||
| Pref1 | Forward | 5′-GACAGGCCATCTGCTTCACC-3′ | 116 |
| Reverse | 5′-GTTGTAGCGCAGGTTGGACA-3′ | ||
| TNFα | Forward | 5′-caacgccctcctggccaacg-3′ | 114 |
| Reverse | 5′-tcggggcagccttgtccctt-3′ | ||
| 18s | Forward | 5′-GATCCATTGGAGGGCAAGTCT-3′ | 79 |
| Reverse | 5′-AACTGCAGCAACTTTAATATACGCTATT-3′ |