Fig. 3.

CDX2 overexpression increased DRA mRNA and protein levels in Caco-2 cells. A and B: AMAXA was used to transfect Caco-2 cells with the mammalian p-CMV expression vector encoding CDX2. At 48 h posttransfection, CDX2 overexpression was confirmed by measurement of its mRNA level (with GAPDH as internal control) and by densitometric analysis of band intensities to determine protein level. C and D: CDX2 overexpression significantly increased DRA mRNA and protein levels. Values are means ± SE of 3 independent experiments. *P < 0.05; **P < 0.01.