Fig. 7.

Effect of cAMP stimulation on transepithelial fluxes of sulfate ($J^{\text{SO}4}$) and chloride ($J^{\text{Cl}}$) across the distal ileum from wild-type (WT) and DRA-knockout (KO) mice. Unidirectional sulfate fluxes (A and B) and chloride fluxes (C and D) were measured simultaneously across isolated, short-circuited segments of the distal ileum in standard bicarbonate buffer in response to bilateral application of forskolin (10 μmol/l) and IBMX (100 μmol/l). E and F compare the responses of short-circuit current (I_sc) and transepithelial conductance (G_T). Each data point represents mean ± SE of n = 8 and 7 tissues taken from WT and DRA-KO mice, respectively. *Statistically significant difference (P ≤ 0.05) as determined by one-way repeated-measures ANOVA, followed by Holm-Sidak pairwise comparisons with the preceding control period (0–30 min). M, mucosal; S, serosal.