Figure 4.

ACT‐246475 and ticagrelor dose dependently caused surgical blood loss after standardized punch biopsy of the spleen. Each study drug was administered by continuous infusion to rats to achieve low‐, intermediate‐, and high‐level inhibition of platelet aggregation. Rats were treated with vehicle, with low, intermediate (Mid), and high dose of ACT‐246475 (0.06, 0.2, 0.6 μL/kg/min) and with low, intermediate (Mid), and high dose of ticagrelor (2, 6, 20 μL/kg/min). After surgical wounding of the spleen, blood was collected for 30 min and the weight of lost blood was determined. Data are presented as means ± SEM, n = 9–35. *P < 0.05, **P < 0.01.