Table 1.

Antithrombotic effects of P2Y₁₂ antagonists in FeCl₃ model in rats

ACT‐246475 infusion (μg/kg/min)	0	0.06	0.2	0.6
Mean area of vascular lumen occluded by thrombi (n = 4)	100%	52%	29%	20%
Blood flow (means ± SEM) n = number of rats	0% n = 35	25 ± 9% n = 9	65 ± 16% n = 10	104 ± 2% n = 10
Patency of carotid artery
Open	0/35	0/9	6/10	10/10
Partially occluded	0/35	4/9	1/10	0/10
Occluded	35/35	5/9	3/10	0/10
Ticagrelor infusion (μg/kg/min)	0	2	6	20
Mean area of vascular lumen occluded by thrombi (n = 4)	100%	94%	37%	19%
Blood flow (mean ± SEM) n = number of rats	0% n = 35	17 ± 5% n = 11	84 ± 12% n = 11	111 ± 4% n = 9
Patency of carotid artery
Open	0/35	0/11	6/11	9/9
Partially occluded	0/35	2/11	4/11	0/9
Occluded	35/35	9/11	1/11	0/9

Quantification of platelet‐rich thrombi. Paraffin sections of the carotid artery thrombi were deparaffinized, rehydrated and stained with hematoxylin and eosin for histopathological and morphometric evaluation of platelet‐rich thrombi. The mean percentage area of occluded vascular lumen was calculated from one section per rat (n = 4). Quantification of blood flow at 30 min. The mean blood flow at the end of infusion (i.e., at 30 min) was quantified and expressed as percentage from baseline blood flow measured in each rat before vessel injury. Assessment of vessel patency. At the end of the infusion, the patency of the carotid artery was designated either as open (no impairment of blood flow), partially occluded (less than baseline blood flow) or occluded (no blood flow).