Figure 9.

The relationship of the IC ₅₀ measured by SPR spectroscopy for the 17 bivalent naphthalimide analogs and the three previously reported compounds (ALE‐0540, PD 90780 and Ro 08‐2750) and the IC ₅₀ measured using the PC12 cell NGF‐dependent differentiation assay. X‐axis: IC ₅₀ (μmol/L) measured using the SPR–Various concentrations of analog were incubated for 1 h with 10 nmol/L NGF prior to injection over immobilized TrkA. Y‐axis: IC ₅₀ (μmol/L) measured following PC12 cell treatment of increasing analog concentration and 500 pM NGF for 72 h. Each data point represents the mean of triplicate results. (F(1,18) = 22.69; P = 0.0002; R ² = 0.5576).