Figure 2.

A short‐term (60 min) preexposure to 17βE necessitates the use of high, pharmacological 17βE concentrations to inhibit depolarization‐induced contractions to KCl. Rings were incubated for 60 min in 1 pmol/L, 1 nmol/L, 1 μmol/L, 10 μmol/L, or 100 μmol/L 17βE, or its EtOH solvent, before generating cumulative concentration–response relationships to KCl. The KCl‐induced tension development is expressed as percent (%) of the maximal contractile response to 80 mmol/L KCl before incubating rings in 17βE or EtOH. The data are shown as the mean ± SE and analysis was conducted using two‐way repeated measures ANOVA (Holm–Sidak post hoc). *P < 0.05, 10 μmol/L or 100 μmol/L 17βE versus EtOH at the same KCl concentration (n = 5 pigs).